mouse antihuman antibody il 6  (InvivoGen)

Journal: Chembiochem : a European journal of chemical biology

Article Title: Gucosamine-6-sulfamate Analogs of Heparan Sulfate as Inhibitors of Endosulfatases

doi: 10.1002/cbic.201000401

Figure Lengend Snippet: (A) Biochemical evaluation of inhibitors against Sulf-1 and Sulf-2 using 4-MUS assay. Bars: percent inhibition in the presence of 5 mM inhibitor relative to uninhibited controls (*3a and 3b show full inhibition at 5mM, and bars are labelled with half maximal biochemical activity, or IC50, values). (B) Selectivity of sulfamate inhibitors. GlcN-6-sulfamates 3a and 3b are more selective for the Sulfs than PARS and are more potent than general inhibitors 1 and 2 (Bars: percent activity in the presence of 500µM inhibitor relative to uninhibited control). (C) Anti-inflammatory activity of Inhibitors. Production of IL-6 by IL-1β stimulation is a proinflammatory response in arthritis. Data show this response is inhibited by up to 50% by sulfamate-based sulfatase inhibitors (Bars: percent the IL-6 response is attenuated in the presence of 200 µM inhibitor relative to uninhibited control in IL-1β stimulated fibroblast-like synoviocytes).

Article Snippet: Half-area ELISA plates (Costar, Cambridge, MA) were coated with 25 µL of a 2 µg/mL monoclonal mouse antihuman IL-6 capture antibody (R & D Systems) for 4 hours and blocked with 1 % BSA.

Techniques: Inhibition, Activity Assay

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Degenerative IVD conditioned media and acidic pH sensitize sensory neurons to cyclic tensile strain

doi: 10.1002/jor.24682

Figure Lengend Snippet: Blocking IL-6 abolishes degenerative IVD induced elevated neuron activity in response to mechanical stimulation. A, The percentage of rat DRG neurons exhibiting calcium transients as a function of strain fitted to a Boltzman curve for the control media, degenerative IVD conditioned media and degenerative IVD conditioned media supplemented with IL-6 blocking antibody (n = 5 patients) groups. B, The maximum calcium transients of rat DRG neurons exposed to mechanical strain in the presence of control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. C,D, The S50 (C) and Smax (D) generated from dose-response curve fitting for the control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. A, Values are mean ± SD. *P < .05 compared to control media group at same strain level. Error bars are SD. B-D, Values are mean ± SD. *P < .05 compared to control media group. Error bars are SD. DRG, dorsal root ganglia; IL, interleukin; IVD, intervertebral disc

Article Snippet: Based on previous experience with IVD conditioned media, 32 mouse monoclonal antihuman IL-6 antibody (Life Technologies) was added to degenerative IVD conditioned media and incubated at 37°C for three hours prior to addition to neurons as described in the Degenerative IVD Conditioned Media Exposure section ( n = 5).

Techniques: Blocking Assay, Activity Assay, Generated

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Degenerative IVD conditioned media and acidic pH sensitize sensory neurons to cyclic tensile strain

doi: 10.1002/jor.24682

Figure Lengend Snippet: Blocking IL-6 abolishes degenerative IVD induced elevated neuron activity in response to mechanical stimulation. A, The percentage of rat DRG neurons exhibiting calcium transients as a function of strain fitted to a Boltzman curve for the control media, degenerative IVD conditioned media and degenerative IVD conditioned media supplemented with IL-6 blocking antibody (n = 5 patients) groups. B, The maximum calcium transients of rat DRG neurons exposed to mechanical strain in the presence of control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. C,D, The S50 (C) and Smax (D) generated from dose-response curve fitting for the control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. A, Values are mean ± SD. *P < .05 compared to control media group at same strain level. Error bars are SD. B-D, Values are mean ± SD. *P < .05 compared to control media group. Error bars are SD. DRG, dorsal root ganglia; IL, interleukin; IVD, intervertebral disc

Article Snippet: IL-6 blocking experiments Based on previous experience with IVD conditioned media, 32 mouse monoclonal antihuman IL-6 antibody (Life Technologies) was added to degenerative IVD conditioned media and incubated at 37°C for three hours prior to addition to neurons as described in the Degenerative IVD Conditioned Media Exposure section ( n = 5).

Techniques: Blocking Assay, Activity Assay, Generated

Journal: Chembiochem : a European journal of chemical biology

Article Title: Gucosamine-6-sulfamate Analogs of Heparan Sulfate as Inhibitors of Endosulfatases

doi: 10.1002/cbic.201000401

Figure Lengend Snippet: (A) Biochemical evaluation of inhibitors against Sulf-1 and Sulf-2 using 4-MUS assay. Bars: percent inhibition in the presence of 5 mM inhibitor relative to uninhibited controls (*3a and 3b show full inhibition at 5mM, and bars are labelled with half maximal biochemical activity, or IC50, values). (B) Selectivity of sulfamate inhibitors. GlcN-6-sulfamates 3a and 3b are more selective for the Sulfs than PARS and are more potent than general inhibitors 1 and 2 (Bars: percent activity in the presence of 500µM inhibitor relative to uninhibited control). (C) Anti-inflammatory activity of Inhibitors. Production of IL-6 by IL-1β stimulation is a proinflammatory response in arthritis. Data show this response is inhibited by up to 50% by sulfamate-based sulfatase inhibitors (Bars: percent the IL-6 response is attenuated in the presence of 200 µM inhibitor relative to uninhibited control in IL-1β stimulated fibroblast-like synoviocytes).

Article Snippet: Half-area ELISA plates (Costar, Cambridge, MA) were coated with 25 µL of a 2 µg/mL monoclonal mouse antihuman IL-6 capture antibody (R & D Systems) for 4 hours and blocked with 1 % BSA.

Techniques: Inhibition, Activity Assay